Nanostructured Memristor Sensor Mimics Acetylcholinesterase (ACHE) Active Sites in the Gorge for fM Detection of Acetylcholine

E.T. Chen, J. Thornton, C. Ngatchouand, S-H. Duh
Advanced Biomimetic Sensors, Inc., US

Keywords: nanobiomimetic memristor sensing, acetylcholinesterase (ACHE) gorge, acetylcholine

Summary:

Many diseases including cancer, diabetes, brain injury, epilepsy, Parkinson’s, autism and Alzheimer’s involve the up regulation of acetylcholinesterase (ACHE) inappropriately signaling of cholinergic synapse for an excess production of the neurotransmitter acetylcholine (ACH). Challenges for providing a nonenzymatic detection device for ACH with rapid detection time and high sensitivity are paramount due to time consuming assays and protein interferences. We report two types of nanostructured memristor biomimetic ACHE sensors developed for detection of ACH in order to solve the challenges. Memristor sensor #1 was made for mimicking the normal active sites in the ACHE gorge, and memristor sensor #2 was made for mimicking the mutated gorge for direct detection of ACH without using antibody and no biomarkers. Multiple polymers were cross linked and self-assembled on gold chips forming a “vertical bridged-nanpore ACHE gorge for sensor #2; and formed a “flat-bridge/nanopore gorge for sensor #1. Frequency effects on the i-V curves were observed of hysteresis. Results obtained by an Amperometric (CA) method for quantitation of ACH revealed the sensor #1 has Detection of Limits of ACH with the DOL of 9.7x10-16 M/cm2 over 10-15 to 10-6 M. However, the sensor #2 was unable to detect ACH directly.