Nucleic Acid-Programmed Polymeric Nanomaterials for Biological Communication

A.M. Rush, D.A. Nelles, M-P Chien, M.P. Thompson, G.W. Yeo, N.C. Gianneschi
University of California San Diego, US

Keywords: DNA nanotechnology, DNA delivery, polymeric nanomaterials, stimuli-responsive materials

Summary:

The work herein describes a novel of class of hybrid materials consisting of synthetic DNA-polymer amphiphiles that self-assemble into discrete micellar nanoparticles whose physical and chemical properties depend on both the nature of the DNA sequence as well as the chemical composition of the polymer. These materials address three key concerns with respect to significant challenges within the field of DNA delivery and stimuli-responsive materials. 1) They arrange DNA in a dense array at the nanoparticle surface, protecting the nucleic acid shell from nuclease degradation. 2) They undergo rapid cellular uptake, distribute into cellular cytosol, and hybridize in a sequence-specific fashion to designated mRNA sequences. 3) They are capable of undergoing dramatic morphological changes in response to specific nucleic acid and enzymatic stimuli.