Highly Sensitive Biosensor Device for Monitoring Colorectal Cancer and Early Detection of Myocardial Infarction

C. Yilmaz, M. Asanterabi, V. Torchilin, A. Busnaina
Center for High-rate Nanomanufacturing, US

Keywords: nanoparticles, ELISA, colorectal cancer, myocardial infraction

Summary:

We have developed a low-cost, highly sensitive and multiplex fluorescence enzyme-linked immunosorbent assay (ELISA) based biosensor. The multiple-marker biosensor has the following unique features: (i) fabrication of the nano-patterned micro-chip using high-volume semiconductor manufacturing techniques, leading to a low cost device, (ii) functionalization of nanoparticles (NP) with correct antibody orientation to maximize antigen capture, leading to lower antibody costs to manufacture, (iii) assembly of the functionalized NP to their specific locations employing site specific electrophoresis, enabling a low detection limit, (iv) assembly of multiple types of functionalized NP, allowing simultaneous detection of multiple biomarkers on a single platform, leading to high detection sensitivity and specificity, and (v) use of low sample volume of whole-blood (from a finger prick) and fast assay time (<30 minutes), enabling point of care (POC) testing. Using a simple fluorescence-based enzyme-linked immunosorbent assay (ELISA) technique, the biosensors exhibit high sensitivity, detecting 31.25 pg/mL of carcinoembryonic antigen and 500 pg/mL of cardiac myosin. This demonstrates the potential for a biosensor device for early detection and monitoring recurrences of colorectal cancer and detection of myocardial infarction.