Magnetically driven drug release from Fe3O4-phage network

C.B. Mao
University of Oklahoma, US

Keywords: phage, iron oxide, network, drug release


Networks assembled from engineered filamentous phage and nanoparticles showed a unique cell-targeting ability. It would be highly desirable if such phage-based cell-targeting networks can also carry drugs for medical applications. In addition, it is still a challenge to construct a stimulus-responsive network made of naturally occurring biopolymers, such as phage, instead of widely used synthetic polymers. Here, we reported a novel magnetic field-responsive network assembled from M13 phage nanofibers and Fe3O4 magnetic nanoparticles (MNPs) and the magnetically controlled release of an anti-cancer drug from the network for eradicating MCF-7 breast cancer cells in vitro. Briefly, negatively charged phage nanofibers were electrostatically cross-linked by positively charged Fe3O4 MNPs to form the Fe3O4-phage network structures. In the presence of magnetic field, Fe3O4 MNPs in Fe3O4-phage network aggregated and thereby resulted in the decrease of its pore size and faster release of loaded drugs from the network. The drug releasing rate was positively correlated to the strength of the applied magnetic field. Fe3O4-phage networks without drug showed no cytotoxicity against normal cells. But when loaded with the anti-cancer drug, the Fe3O4-phage networks were able to release the drug to the medium for the eradication of breast cancer cells.