Bio-compatibility of silver nanomaterials using the embryonic zebrafish bioassay

J.A. Fisher, R.L. Tanguay
Oregon State University, US

Keywords: zebrafish, development, embryos, in vivo, heart rate, bioassay, nanomaterials

Summary:

The bio-compatibility of silver nanomaterials (AgNP) is currently of great interest to regulators and health and safety professionals because it is a material that has the potential to reach mass populations. Four well characterized (Harper et al., 2011) silver nanomaterials (AgNP) were obtained in order to investigate low-concentration biological effects using three well-established zebrafish (Danio rerio) bioassays. The assays measure mortality and morbidity (Truong et al., 2011), and behavior (Saili et al., 2011). Here we report the development and implementation of a heart rate assay that requires only 30 seconds per animal using this semiautomated technique. The materials were obtained from the NIEHS Centers for Nanotechnology Health Implications Research (NCNHIR) Consortium and characterized by the National Characterization Laboratory (NCL). The same materials were provided to numerous laboratories conducting parallel in vivo and in vitro studies allowing for comparative analysis.