Green Synthesis of bio-compatible Au nanoparitcles using phytochemicals

J. Lee, H. Kim, J. Kim, D-W Han, K. Koh, J. Lee
Pusan National University, KR

Keywords: green synthesis, bio-compatible Au nanoparticle, anti-oxidant effect, phytochemicals

Summary:

Green chemical synthesis of Au nanoparticles has been of great interest because of their potential biomedical applications. In this study, we successfully produced green chemical Au nanoparticles using the phytochemicals which are gallic acid, protocatechuic acid and isoflavon at room temperature. They have strong anti-oxidant effect, so can serve as a reducing agent. Poly ethylene glycol (PEG) was used polymeric repulsive agents in inter-nanoparticles. The particles are homogeneous with average diameter of 20 nm and high dispersity at all ranges of pH. Absorption measurements indicated that the plasmon resonance wavelength, λmax of Au nanoparticles is 530 nm. The properties of green chemical Au nanoparticles characterized by TEM, UV/Vis spectroscopy, FT-IR and Zeta-potential. Through the cell culture test of green Au nanoparticles, the particles were presented for non-toxicity. Phytochemical-stabilized Au NPs allowed about 40 % H2O2 to be removed at an NP concentration of 50 ug/mL; this removal rate is the same as that achieved by 3000 units/mg catalase. Therefore, this novel synthesis route for Au NPs using phytochemical reducing agents may be effectively exploited for non-thermal-assisted reactions and one-pot processes of biological applications.