Microfluidic Control of Adipose-Derived Stem Cell Growth and Positioning

N. Wadhawan, H. Kalkat, X. Ma, S. Gajjeraman, S. Nandagopal, N. Hao, J. Deng, B. Xiang, M. Zhang, G. Tian, F. Lin
University of Manitoba, CA

Keywords: microfluidic device, adipose derived stem cells

Summary:

Microfluidic devices provide powerful new tools for quantitative stem cell research owing to its advantages in cellular microenvironmental control and experiment throughput over conventional methods. Particularly, microfluidics is playing a growing role in studying stem cell growth, differentiation and trafficking mediated by chemical factors such as different growth and differentiation factors and physical parameters such as direct current electric fields (dcEF). In the present study, we focused on adipose-derived stem cells (ADSCs) and we quantitatively characterized ADSCs growth and positioning mediated by epidermal growth factor (EGF) and dcEF using microfluidic devices. Our results revealed that ADSCs preferentially grow toward the EGF gradient. In addition, we showed that ADSCs tend to orient perpendicularly to the dcEF. These microfluidics-based results demonstrated the interesting roles of EGF and dcEF in controlling ADSCs growth and trafficking.