Cytotoxicity of silver nanoparticles

G.A. Sotiriou, A. Pratsinis, J.-C. Leroux, S.E. Pratsinis
Particle Technology Laboratory, ETH Zurich, CH

Keywords: nanosilver, toxicity, macrophages


Silver nanoparticles are receiving more and more attention for their plasmonic colors and their antibacterial effects [1]. The addition of a nanothin SiO2 shell minimizes the release of toxic Ag ions and the contact of cell or bacteria with the Ag particle surface. [2] Here the development and validation of a cellular test, such as the MTS, to quantitatively estimate/assess the cytotoxicity of various Ag nanoparticles is presented. In this study, the cytotoxicity of silver nanoparticles with different sizes was investigated against macrophages using the MTS assay. The effect of the different nanosilver size and the Ag+ ion release is investigated. This method has the potential to be expanded to other cytotoxic tests, to get a broader understanding of the toxicity of such particles. References [1] Sotiriou, G.A, Pratsinis, S.E. Antibacterial Activity of Nanosilver Ions and Particles. Environ. Sci. Technol.44, 5649-5654 (2010). [2] Sotiriou, G.A, Hirt, A.M., Lozach P.Y., Teleki A, Krumeich F., Pratsinis, S.E. Hybrid, Silica-Coated, Janus-Like Plasmonic-Magnetic Nanoparticles. Chem. Mater.23, 1985-1992 (2011).