Cytotoxicity and genotoxicity evaluations for superparamagnetic iron oxide nanoparticles coated with various functional groups

J. Lee, S. Hong, F. Sun, J. Lee, J. Kim
Pusan National University, KR

Keywords: M. Tb, virus, internalization, nanoparticle

Summary:

Superparamagnetic iron oxide nanoparticles (SPIONs) have been widely utilized for diagnosis and therapy of specific diseases as magnetic resonance imaging (MRI) contrast agents and drug delivery carriers due to their easy transportation to targeted areas by an external magnetic field. A SPION synthesized by Stober method was covered with –O- group, called bare SPION. Then, it was modified with three different types of functional groups such as hydroxyl (–OH), carboxylic (–COOH) and amine (–NH2) via coating of their surfaces with TEOS, APTMS, TEOS-APTMS or citrate, which allowed the particles to have different surface charge and size.The effects of SPIONs coated with these functional groups on the mitochondrial activity, intracellular accumulation of reactive oxygen species, membrane integrity and DNA stability in L-929 fibroblasts were determined by WST-8, DCF, LDH and comet assays, respectively. From consequent toxicological observation, it is probable that the functional groups and sizes of SPIONs are critical determinants of cellular responses, degrees of cytotoxicity and genotoxicity and potential mechanisms of toxicity. However, in general, the surface modified - SPIONs at lower than 500 ppm irrespective of the types of surface functional groups showed neither cytotoxicity nor genotoxicity against the cells.