Engineered Stem Cell Environments: The Path to Therapeutics

A.W. Martin
Corning Incorporated, US

Keywords: synthetic surfaces, stem cells

Summary:

Presentation Title: Engineering Stem Cell Environments – The Path Towards Stem Cell Therapeutics Abstract This presentation describes the development of novel nano-feature size synthetic coatings to support the path forward towards stem cell therapeutics. In other words, can the research in this area be taken from the lab side to the bedside; can we develop stem cell supporting synthetic nano-substrates that can be commercialized? To address these critical questions, the engineering of stem cell environments and the evolutionary path taken towards manufacturing low cost, robust, scalable, sterilized, FDA regulatory friendly and xeno-free synthetic nano-synthetic surfaces are described. Human embryonic stem cells (hESCs) have two properties of interest for the development of cell therapies: self-renewal and the potential to differentiate into all major lineages of somatic cells in the human body. Herein we describe (7) different types of nano-peptide mimetic surfaces that are all related to our current commercialized surface, i.e., Corning® Synthemax™ Surface. Different chemical approaches and the evolutionary path taken in developing peptide-acrylate surfaces for supporting the pluripotency of hESCs and multipotency of hMSCs and hNSCs have been demonstrated. We have also shown the ability of these surfaces to support differentiation.