Continuous-flow polymerase chain reaction (PCR) on a right triangular shaped polydimethylsiloxane (PDMS) microdevice using a single heater

W. Wu, K.T.L. Trinh, N.Y. Lee
Gachon University, KR

Keywords: on-chip polymerase chain reaction (PCR), right triangular prism shaped microdevice, poly(dimethylsiloxane) (PDMS), a single heater, temperature gradient, multiplex PCR

Summary:

In this study, we propose the fabrication of a right triangular prism shaped microdevice and apply the device for performing an on-chip continuous-flow PCR employing a single heater. Consistent temperature control employing two or more heaters is one of the main obstacles of current PCR system, which limits the miniaturization of the device. The proposed triangular prism shaped device, when combined with the low thermal conductivity of the silicone polymer material, in this case poly(dimethylsiloxane) (PDMS), creates a height-dependent temperature gradient on its slanted plane from the use of a single heater. The feasibility and the universal applicability of the proposed microdevice were examined by amplifying multiple templates at varying inclination angles of the device, and the potential of performing a multiplex PCR using a single device was also explored. A 409 bp lung cancer biomarker and a 230 bp plasmid vector were successfully amplified in less than 30 min on a single microdevice simultaneoulsy, paving the way for a microscale, multiplex PCR on a single platform employing a single heater.