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One Pot vs Two Steps Synthesis of Fluorescent Gold Nanoparticles and Capping with Thiolated Organic Linker

S.K. Rastogi, B.D. Denn, A.L. Branen
University of Idaho, US

Keywords: one pot, gold nanoparticles, thiolated linkers, fluorescence, TEM spectroscopy


Nobel metal nanoparticles (NPs) such as Au and Ag have gained great attention in the past decades due to their fundamental optical properties. Based on that interest, the current work is focused for the synthesis and characterization of fluorescent gold nanoparticles (F-AuNPs) from hydrogentetrachloroaurate(III) trihydrate, using one pot or two steps approaches and capping with thiolated organic linkers, in N,N’dimethylformamide (DMF). Where, DMF serves as a mild reducing agent at high temperature as well as solvent for the reaction and five different thiolated organic linkers with second functional group of carboxylic acid, hydroxyl, and aromatic amine, were used. The capped F-AuNPs show fluorescence emission intensity at 425±5 nm upon excitation of 330±5 nm in organic and in aqueous suspension. The F-AuNPs were characterized using ultraviolet visible absorption spectroscopy, fluorescent emission spectroscopy, fluoro-count reader, Fourier transform infrared spectroscopy, dynamic light scattering analysis, and transmission electron microscopy. The F-AuNPs would have a wide application in labeling, imaging and sensing of specific nucleic acid and protein for developing diagnostic or therapeutic tool.
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