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Nanostructural Effects of Mitomycin C Applications on Scleral Collagen Fibrils: Atomic Force Microscopy Observation

S. Choi, Y. Cheong, E. Kwon, G.B. Jung, H.-J. Lee, J.-H. Shin, K.-H. Jin, H.-K. Park
Kyung Hee University, KR

Keywords: atomic force microscopy, human sclera, collagen fibrils, Mitomycin C, diameter and D-periodicity

Abstract:

This study was to investigate the effects of Mitomycin C on the scleral collagen surfaces by atomic force microscopy (AFM). Two non-contact mode AFM machines were used to observe changes in the morphological characteristics of human scleral surfaces before and after 0.02% Mitomycin C application for 1, 3 and 5 minutes. From AFM topography and deflection images of the collagen fibril, the morphological characteristics of scleral fibrils including the fibril diameter and D-banding were measured using line profile. The sclera collagen fibril treated with 0.02% Mitomycin C for 1 min showed no significant increase in mean fibril diameter (155.04 ± 17.46 nm) and the mean D-periodicity (70.02 ± 3.33 nm), compared to the control group. However, the scleral collagen fibrils treated with 0.02% Mitomycin C for 3 and 5 min showed a significant increase in mean fibril diameter (182.33 ± 16.33 nm and 199.20 ± 12.40 nm) and mean D-periodicity (70.27±13.66 nm and 72.75 ± 19.32 nm), compared to the control group. This study examined the structural changes in the scleral collagen fibrils before and after Mitomycin C application by AFM technique. The results indirectly suggest that long-term Mitomycin C application might increase the incidence of complications like a scleromalcia.
 
 
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