TechConnectWorld 2011 Conference and Expo TechConnect World 2011 Nanotech 2011 Clean Technology 2011 Microtech 2011 Bio Nanotech 2011 TechConnect Summit and Expo 2011

Polymersomes and Imaging: An Quantitative Approach

A.S. Joseph, N.J. Brown, G. Battaglia
University of Sheffield, UK

Keywords: Polymersomes, fluorescence, HPLC, imaging

Abstract:

Nano-sized carriers are widely used as imaging tools. Quantification of the amount of carrier delivered provides useful informations in development and testing stages. This work describes a HPLC method to quantify fluorescent labelled polymersomes. Polymersomes are nanometer-sized vesicles made by the self-assembly of amphiphilc co- polymers in water . We use pH sensitive PMPC25-PDPA70 and PEO113 -PDPA70 co-polymers labelled with Rhodamine 6G. We analyse interactions of these two formulations with different cell populations using quantitative fluorescence microscopy imaging, fluorescence activated cell sorting (FACS) and HPLC quantification. A method is here developed to quantitatively recover and quantify polymersomes from cell lysate. Cellular studies are performed in static and in flow conditions, using mouse fibrosarcoma cells over-expressing different isoform of VEGF. Epifluorescence analysis showed that overtime there is an increase of up-take for both formulations. FACS analysis confirmed this trend . A significant difference was found between the two formulations in terms of percentage of cells up-taking fluorescent labelled polymersomes. The HPLC method we developed confirmed this difference and it also allowed to measure the quantity of labelled polymers up-taken. These results will provide the basis for the development of vesicles with targeting moieties.
 
 
Sessions Monday Tuesday Wednesday Thursday Authors Keywords Affiliations Search

TechConnect World 2011 Nanotech 2011 Clean Technology 2011 Microtech 2011 BioNanotech 2011 TechConnect Summit 2011
2011 Events | Program | Speaker | Short Courses Sponsor Exhibit/Showcase Press Venue Register
Subscribe | Contact | Site Map
© Copyright 2010 TechConnect World. All Rights Reserved.