TechConnectWorld 2011 Conference and Expo TechConnect World 2011 Nanotech 2011 Clean Technology 2011 Microtech 2011 Bio Nanotech 2011 TechConnect Summit and Expo 2011

The mechanical sensitivity of vesicle dynamics in in vivo and in vitro neurons

W. Ahmed, T. Li, S. Rubakhin, A. Chiba, J. Sweedler, T.A. Saif
University of Illinois at Urbana-Champaign, US

Keywords: cell mechanics, neuron, vesicle, mechanotransduction, stretching device

Abstract:

To study the mechanical response of both in vivo and in vitro neurons, we have developed a novel platform for in-situ high-resolution live-imaging of cells and tissues under applied mechanical strain. The system is capable of applying tensile and compressive strain (up to 45%) as was characterized experimentally by digital image correlation, and computationally by the finite element method [W. Ahmed et al Acta Biomat 2010]. Our system allows dynamic studies of cellular and subcellular structures in cells and tissues with high spatial and temporal resolution. Here we present the results of two new investigations: (1) Tension induced synaptic vesicle accumulation in in vivo Drosophila motor neurons. (2) Compression induced disruption of vesicle transport in in vitro Aplysia neurons. Here we investigated the subcellular dynamics of in vitro and in vivo neurons in response to mechanical stimulation to study the role of mechanics in neuronal function. Our system allows studies of living cells by direct observation of subcellular structures under precise mechanical stimulation, which will contribute to elucidating the underlying molecular mechanisms of mechanically induced biochemical signaling.
 
 
Sessions Monday Tuesday Wednesday Thursday Authors Keywords Affiliations Search

TechConnect World 2011 Nanotech 2011 Clean Technology 2011 Microtech 2011 BioNanotech 2011 TechConnect Summit 2011
2011 Events | Program | Speaker | Short Courses Sponsor Exhibit/Showcase Press Venue Register
Subscribe | Contact | Site Map
© Copyright 2010 TechConnect World. All Rights Reserved.