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A sensitive MR method for DNA detection with magnetic nanoparticles.

D. Alcantara, Y. Guo, H. Yuan, L. Josephson
MGH-Harvard Medical School, US

Keywords: nanoparticles, T2, magnetic relaxation switch, magnetic nanoparticles

Abstract:

We hypothesized that a magnetic nanoparticle (NP) surface functionalized with multiple DNA binding fluorochromes might react with DNA through a multivalent, avidity-type reaction and yield a highly sensitive method for detecting DNA by the T2 relaxation time of water protons measured by Magnetic Resonance (MR) techniques. Although oligonucleotides have been used to target NP’s to specific sequences on nucleic acids, the considerable literature on fluorochrome/nucleic acid interactions has not been mined for potential nanoparticle (NP) targeting strategies. Our study showed how a NP displaying a DNA-binding fluorochrome, bind in a multivalent fashion to yield a highly sensitive, T2-based method of detecting DNA.
 
 
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