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Human Mesenchymal Stem Cells (hMSCs) Interaction with Nanoporous Titania Substrates

D.K. Nagesha, J.T. Maniscalco, D. Guarnieri, V. Belli, P.A. Netti, S. Sridhar
Northeastern University, US

Keywords: Human Mesenchymal Stem Cells, titania, nanoporous surface, cell interaction, adhesion


Nanoporous titania (TiO2) are increasingly being investigated for biomedical applications ranging from coatings on cardiovascular stents to dental implants and artificial metallic joints. An elegant and established method to fabricate nanoporous TiO2 is through the controlled anodization of Ti in an electrolyte containing fluoride or chloride ions. The dimension of TiO2, pore size and thickness can be precisely controlled by varying the experimental conditions. The pore lengths in the order of several microns can be uniformly fabricated using chloride ions in the electrolyte. The nanoporous feature of these TiO2 surface allows for their use as drug reservoir for localized drug delivery. In addition, the nano-topographical features have remarkable influence on the proliferation and adhesion of cells. In this work, we studied the interaction of human mesenchymal stem cells (hMSCs) with nanoporous TiO2 surface of different sizes. The cell morphology and cytoskeletal organization was followed by observing the stained actin microfilaments. When compared to bare TiO2 surface, nanoporous TiO2 showed an enhanced cell adhesion and a striking difference in cell stretching and polarization. Furthermore, it was observed that nanofeatures on TiO2 surface also influence the migration paths of hMSCs suggesting the presence of topological contact guidance skin.
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