Highly sensitive detection of cancer biomarker EGFR by PicoTREC-SERS

L. Xiao, S. Harihar, Y. Wu, D.R. Welch, A. Zhou
Utah State University, US

Keywords: epidermal growth factor receptor, breast cancer metastasis suppressor 1 (BRMS1) gene


It is reported that for women in the U.S., breast cancer death rates are higher than those for any other cancer, besides lung cancer. Considering the fact that approximately 70–80% of breast carcinomas overexpress the epidermal growth factor receptor (EGFR)1 and that the cell surface EGFR represents a current major therapeutic target to treat breast cancer (i.e., anti-EGFR therapy treatment), there is an urgent need to develop an ultrasensitive technique to detect EGFR at the single molecule level on breast cancer cells. Up to now, it’s not clear about spatial distribution and/or distribution pattern of EGFRs on the single cell level. To achieve this goal, atomic force microscope (AFM) PicoTREC and surface enhanced Raman spectroscopy (SERS) were applied in the present work.