Covalent Funtionalization for Multi-walled Carbon Nanotube (f-MWCNT)-Folic Acid bound Bioconjugate

J.M. Ngoy, S.E. Iyuke, E.W. Neuse, C.S. Yah
University of the Witwatersrand, ZA

Keywords: functionalized multi-walled carbon nanotubes, functionalized carbon nanotubes folic acid, bioconjugates, nanocomposites

Summary:

Folic acid (FA) is an essential, bioavailable water soluble B-complex vitamin that helps in the replication of normal cells and it deficiency can leads to oncogenic cells development. The objective of this work was to synthesize new water-soluble bioconjugate carrier which in conjugation to anti-cancer drugs improves drug selectivity and decreases negative side effects. Folic acid shows a great deal of promise as a cancer-homing agent and can also be used as a coenzyme for cells restoration. The synthesis of bioreversible binding to water-soluble and biocompatible carbon nanotube is an approach to circumvent critical pharmacological and efficacious biological action. Carbon nanotube (CNT) obtained via catalytic chemical vapour deposition was covalently functionalized at RT, 50ºC and 100ºC with a mixture of sulphuric and nitric acids to generate the phenol and carboxyl groups on the surface. The increase of temperature caused the decrease of mol ratio (COOH/OH) relevant to the increase of water solubility respectively. Using a diamine, both terminal carboxyl of f-CNT and FA was attached respectively to each terminal amine through an active ester to produce f-CNTs-FA conjugate. After H NMR, FTIR and TEM, 100 % incorporation of FA, CONH as bioreversible bond and nanotube structure have been evaluated respectively.