Incorporation of Gold Nanoparticles into MCF-7 Breast Cancer Cells in the Presence of Estradiol

C. Lara-Cruz, L. González-Núñez, J.E. Jiménez Salazar, R.C. Lazzarini-Lechuga, E. Ramón-Gallegos, P. Damián-Matsumura and N. Batina
UAM-Iztapalapa, MX

Keywords: AFM, estradiol, nanoparticles


Variations in membrane roughness are related with AuNP therapy. Estradiol (E2) can modulate membrane fluidity; however, this largely remained uninvestigated in the presence of AuNP. We detected changes in the morphology and physical properties of individual human breast cancer MCF-7 cells by atomic force microscopy (AFM) and visualized AuNP (20 nm) intracellular localization by confocal microscopy, in the absence and presence of E2. AFM shows changes in MCF-7 plasma membrane roughness ( the RMSRq value), with maximum effect (22%) observed after 12 hours of incubation with E2 (1 nM). During the incubation with AuNP (20 nm; 80 μg/mL) in combination with E2, plasma membrane roughness increased after 6 hours. AuNP were localized in the cytoplasm even at 2 hours of incubation in the presence of E2; meanwhile, in the absence of E2 this process takes up to 12 hours. Uptake and transport of the AuNP in breast cancer cells was mediated by lysosomes, by confocal laser scanning microscopy. AuNP exerted concentration-dependent MCF-7 cell cytotoxicity at 80 μg/mL and 72 hours of incubation, and was enhanced in the E2 presence. It provids new insight for combined nano-hormonotherapy of the human breast carcinoma.