Ciliated micropillar array for nanoscale lipid vesicle isolation

Z. Wang, D. Fine, H. Wu, J. Schmulen, Y. Hu, B. Godin, J. Zhang, X. Liu
the University of Texas at Austin, US

Keywords: silicon nanowire, hierarchical structure, microfluidics, liposome, exosome

Summary:

We fabricate a microfluidic device consisting of ciliated micropillars, the porous silicon nanowires-on-micropillar structure. We demonstrate that the prototype device can preferentially trap exosome-like lipid vesicles, while simultaneously filtering out proteins, and cell debris. Trapped lipid vesicles can be recovered intact by dissolving the porous nanowires in PBS buffer.