A novel mechanism for the synthesis of ultra-purified silver nanoparticles for the use in biomedical materials

P. Zuniga, S.J. Chaves, J.S. Chaves
Universidad de Costa Rica and Instituto Tecnologico de Costa Rica, CR

Keywords: AgNPs, biocompatibility, biopurification, purity, synthesis


Biomedical products are regulated by high standards of purity and quality compared to those that are not going to be linked to the human body. So it is a real challenge to incorporate a nanotechnological product to be used in the biomedical industry without affecting its cost. Available reagents for the synthesis of AgNPs contain metal impurities such as iron that cannot only reduce the antimicrobial properties but even promote the growth of microbiological agents according to the level of purity of the reagent.In this study, we describe a novel and more affordable mechanism for the synthesis and bio-purification of AgNPs that contain metal impurities. After tests with some microorganisms we have found Pseudomonas aeruginosa as the proper species to the bio-purification and thus obtaining a higher purity of AgNPs.With this novel mechanism, not only we were able to use a reagent with a higher quantity of impurities and reduce the synthesis cost, but also were able to achieve a higher purity in the synthetized nanoparticles.By synthetizing AgNPs this way, we not only obtained nanoparticles with a higher purity than the ones produced by using a higher purity reagent, but also reduced the cost around 50 percent.