Effects of ZnO nanoparticles and ZnCl2 solution on rat liver and kidney

I. Ben Slama, S. Amara, N. Rihane Ben Younes, I. Mrad, K. Omri, J. El Ghoul, L. El Mir, K. Ben Rhouma, H. Abdelmelek, M. Sakly
Faculty of Sciences, TN

Keywords: ZnO nanoparticles, ZnCl2, liver, kidney, rats

Summary:

Recent studies have shown that Zn2+ is released from the surface of ZnO NPs when they are suspended in an aqueous state.The aim of this study was to evaluate the effects of ZnO nanoparticles (ZnO-NPS) and ZnCl2 in solution on plasmatic biochemical parameters, tissues trace elements and their possible cytotoxicity in rat liver and kidney. Rats were treated orally with doses of (ZnO-NPs) or (ZnCl2) (10 mg/Kg) for 5 consecutive days,control group received a dose of 0.9℅ sodium chloride. Sub-acute exposure to ZnO-NPs or ZnCl2 showed no significant change either in body weight, kidneys and liver weight. Oral administration of ZnO-NPs or ZnCl2 increased the plasmatic transaminase activity (AST and ALT). However, the creatinine, uric acid and blood glucose concentrations remained unchanged. Rats received ZnO-NPs or ZnCl2 solution showed a no significant increase of zinc content in the liver and kidney. Morover, the concentration of trace elements were slightly modulated after ZnO-NPs or ZnCl2 exposure. Histological analysis in the liver and kidney showed signs of discreet cytotoxicity (Inflammatory response, Vascular congestion, edema formation) in ZnO-NPs and ZnCl2 treated-rats compared to control group. Oral administration of ZnO-NPs or ZnCl2 showed no obvious toxic effect in rat liver and kidney.