Cystine-functionalized superparamagnetic nanoparticles effect on human immune cells ex vivo

S. Dolci, R. Manetti, G. Pampaloni, F. Sgarrella, C.A. Veracini, V. Domenici, L.G. Delogu
University of Sassari, IT

Keywords: superparamagnetic nanoparticles, immune system, activation

Summary:

We present new cystine-functionalized SPIONs (Cy-SPION) and we investigate their impact on a wide variety of human immune cells ex vivo (T and B lymphocytes, NK cells and monocytes). The relatively cell percentage and cell functionality are evaluated in presence of different doses of Cy-SPIOs (50, 100, 200 ìg/ml). After SPIO treatment and PBS washing, a possible % modulation on PBMC was mesured. PBMC % was found not affected by 50, 100 and 200 ìg/ml of Cy-SPIONs treatment. To assess the possible impact on cell functionality, major immune cell populations were identified by flow cytometry according to the expression of specific cell surface markers: CD4 and CD8 for T cells, CD20 for B cells, CD14 for monocytes CD56 for NK cells. We focused on the major activation markers (CD25, CD69,CD30). CD25 assay showed an increased activation of monocytes not dose dependent. Other histograms show SPIONs didn’t impact cell activation and therefore preserve the functionality of immune cells. Our results, on human cells ex vivo, showed that Cy-SPIONs in different doses didn’t affect the PBMC %. This study encourage the further studies on SPIONs for biomedical applications either as diagnostic nanosystems by intravenous injection or as possible drug carriers.