Development of rapid aptasensor using graphene oxide and 1,1’-oxalyldiimidazole chemiluminescence detection

J. Kwun, S. Yun, J.H. Lee
Luminescent MD, LLC, US

Keywords: aptasensor, graphene oxide, chemiluminescence, food-borne pathogens

Summary:

Highly selective and sensitive aptasensor capable of rapidly quantifying Vibrio parahaemolyticus in the presence of Vibio cholera and Vibrio vulnificus was developed using an specific DNA aptamer, graphene oxide and 1,1’-oxalyldiimidazole chemiluminescence (ODI CL) detection. A sample containing three different Vibrios was mixed with the aptamer conjugated with TEX615 in TE buffer (pH7.5). Then, complexes of Vibrio parahaemolyticus and aptamer were formed in the mixture incubated for 5 minutes. After the incubation, graphene oxides in PBS (10 mM, pH7.4) were added in the mixture to remove aptamers unbound with Vibrio parahaemolyticus within 1 minute. Trace levels of Vibrio parahaemolyticus in the mixture were rapidly quantified when ODI CL reagents (e.g., ODI, H2O2) were inserted. The limit of detection (LOD = average background + 3 × standard deviation) of aptasensor having excellent accuracy, precision, and recovery was 2400 cells/ml. Based on the results, it is expected that highly selective and sensitive aptasensors capable of rapidly quantifying and monitoring food-borne pathogens can be developed using the combination of graphene oxide and ODI CL detection.