A Nanopore Biomimetic Device Quantitatively Detects Early Stage Cancer Cells; a Contour Map Multiple Variable Correlation Method Assesses the Heat of Cancer Cells Released

E.T. Chen, Y. Shen, J. Thornton, C. Ngatchou, S-H. Duh, P.T. Kissinger
Advanced Biomimetic Sensors, Inc., US

Keywords: nanopore biomimetic membrane, ratio of action potential and resting potential, contour map multiple variable correlation method, quantitatively detects early stage cancer cells, heat release

Summary:

Detecting low abundance cancer cell is challenged by the complex instrumentation and time consuming assays. We report a unique cancer cell detection device that is capable of detecting low abundance MDA-MB-231 breast cancer cells based on a nanopore biomimetic cross linked polymer membrane fabricated on gold chips. The breast cancer cells were detected by changes of an action potential when receptor of the cancer cell directly interacts with the active site of the Au/membrane. This change of action potential is recorded using a double step chronopotentiometry (DSCPO) method under labeling-free and antibody-free conditions. Results were compared with a normal breast tissue that did not have interaction. This study was built upon our previous work using nanopore sensor to measure glucose:(Nanotech (3),101-104, 2011). The results show a significant difference of released heats between 5 cancerous cells and a normal breast tissue (0.010 calorie vs. 0.001 calorie). The difference was positively correlated with the high abnormal ratio of action/resting potential as discharge current rose to mA level. The sensitivity of the device is 320 mV/cell.mL-1 corresponding to 5 cell/mL. Effect of cell concentration (1-200 cell/mL) on action and resting potential profiles is reported.