Self assembled and organized invertase aggregation

J.H. Lee, E.J. Kim
Chosun University, KR

Keywords: self assembled, enzyme aggregation, thin layer

Summary:

Self-aggregated enzymes have gained a lot of attention recently, due to their interesting applications and nanostructures. Self-assembly methods to organize biomolecules were initiated by nature and were used to organize biomolecules into a useful form. Ordered assemblies of biomolecules are thus an interesting class of biomaterials that provide exceptional potentials to achieve one-two-and three-dimensional structures. These systems are applicable to various applications such as biotransformations, bioseparation, and biosensors which needs uniform morphology. Surface coating with enzymes and the morphology of enzyme coated surface has been studied in this research. The self assembled and organized enzyme monolayer has been established by self aggregation of enzymes with glutaaldehyde and polyaniline nanofiber. The self-assembly capability of invertase increased with the increase of glutaaldehyde concentrations,. There was optimal polyaniline concentration (15% of aniline concentration for making polyaniline) to form invertase self assembly and optimal enzyme concentration was 10 mg/ml for making stable invertase coated matrix. The developed uniform surface coating can be used for biosensor and biocatalyst.