Controlling the Intracellular Distribution of Agents with Core/Shell Particles Made from Particle Blends

K.K. Tran, H. Shen
University of Washinton, US

Keywords: antigen delivery, polymer blends, intracellular trafficking

Summary:

The ability to deliver agents into multiple intracellular compartments and then target multiple signaling pathways within a single construct can address many challenges in mediating and/or correcting functions of cells. In this study, we developed a novel intracellular delivery platform, polymer blend particles, by utilizing two functionally unique polymers, poly(lactide-co-glycolide) (PLGA) and pH-responsive poly(dimethylaminoethyl methacrylate-co-propylacrylic acid-co-butyl methacrylate) (DMAEMA-co-PAA-co-BMA). The fabrication strategy resulted in polymer blend particles with defined and tunable compositions and core-shell morphology, which enabled the controlled delivery of cargos into the cytosolic space without complete disruption of endosomes. Protein antigen encapsulated in blend particles was delivered to both endosomal compartments and the cytosol. The blend particles offer a facile platform to control the intracellular delivery of agents and engage multiple signaling pathways within a single construct.