Fabrication of Microelectrodes on Polyester Membranes for Dielectrophoretic Cell Capture

C. Hanke, P.S. Dittrich, D.R. Reyes
National Institute of Standards and Technology, US

Keywords: polyester (PET) membrane, dielectrophoresis, gold microelectrodes, metallization, polyelectrolyte mulitlayer, cell capture, microfluidics

Summary:

We present a new system for cell capturing on porous polyester (PET) membranes using dielectrophoretic forces. For the first time gold microelectrodes were fabricated on PET membranes using conventional photolithographic techniques. Their characterization revealed no physical differences before and after processing. Dielectrophoretic cell trapping was successfully carried out within this microfluidic system and cells remained viable at least a day after capturing.