Nanotech 2011

Capacitance-based real time monitoring of three kinds of endocytosis

R.M. Lee, P. Kim, K. Kim, K.-M. Kim, D. Kim, C.O. Yun, J.-S. Shin, K.-H. Yoo
Yonsei University, KR

Keywords: capacitance sensor, receptor mediated endocytosis, pinocytosis, phagocytosis

Abstract:

There exist three kinds of endocytosis; receptor mediated endocytosis, pinocytosis and phagocytosis. First, receptor-mediated endocytosis is essential for targeted gene/drug delivery to a specific cell type. Second, pinocytosis is the engulfing and digestion of dissolved substances and phagocytosis is the engulfing and digestion of microscopically visible particles. In this study, we developed a capacitance sensor to monitor and distinguish three kinds of endocytosis in real time. The capacitance sensor is fabricated simple photolithography process on glass substrate and target cells are positioned between gap electrodes. The capacitance sensor was able to detect a capacitance peak in different cell lines during the internalization of adenoviruses or antibodies via receptor-mediated endocytosis. In contrast, the capacitance declined without a capacitance peak when nanoparticles were taken up via non-specific pinocytosis. In case of phagocytosis, the capacitance shows a peak during the engulfing of bacterium, and then capacitance is abruptly increases due to the competition between engulfing the bacterium and bacteria dividing. Thus, our capacitance sensor represents a potential capacitance-based means of discrimination among receptor-mediated endocytosis, non-specific pinocytosis and phagosytosis. Moreover, we developed a capacitance sensor array to demonstrate capacitance-based high-throughput screening
 

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