Nanotech 2011

Preparation, Characterization and Antioxidative Activity of Anti-aging Nanoemulsion and Lipid Nanoparticles

Q. Xia, H. Wang, J. Tang, J. Wang
Southeast University, CN

Keywords: bio nano materials, lipid nanoparticles

Abstract:

Nanoemulsions (NEs) and lipid nanoparticles (LNs) were regarded as suitable nanosized carriers of water-insoluble active components. These nanosized carriers could be used to load with drugs, nutrients and cosmetic actives and provide the appropriate application approach for the active compound which was very good but unable to be used due to some limitations. Alpha Lipoic Acid (ALA), Coenzyme Q10 (CoQ10) and Vitamin E (VE)are practically insoluble and chemically labile in water and should be incorporated in some carriers such as NE or LN to improve their stability and realize the final application as a versatile anti-oxidants. Both NEs and LNs were prepared by HPH (High Pressure Homogeneity) based on the phase behavior results obtained from the phase diagram. The stability results were obtained using several factors such as temperature, storage time, pH, dilution and high speed centrifugation. The good physical and chemical stability of particles was proved by Photon Correlation Spectroscopy (PCS) and UV/HPLC for different storage times up to two years. UV /HPLC measurements showed that the loading capacity of active after production could reach 10% for ALA, 8% for CoQ10 and 10% for VE. The high entrapment efficiency (nearly 100%) was obtained for all formulations. To elucidate the antioxidative activity of ALA-NLC and CoQ10-NLC, colorimetric method was employed to value the scavenging effects of Hydroxyl radicals, and using the 2, 2-diphenyl-1-picrylhydrazil(DPPH) radical scavenging assay to analyze the cell-based antioxidative activity. The results revealed that active substance loaded LNs expressed antioxidant activity at a similar magnitude as pure active substance. The production line with 100 KG per batch was founded to meet the needs of real industrial application. It includes the operation module, pre-and post- treatment module and assistant module with electric control all the process. The study of industrialization showed that it is possible to realize the industrial production using this production line to obtain the same products as it from labs.
 

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