Nanotech 2010

Determination of the Electroporation Threshold for pulses from 95 ns to 20 us

G. Saulis, S. Balevicius, R. Saule, V. Stankevic, N. Zurauskiene
Vytautas Magnus University, LT

Keywords: nanoporation, mouse hepatoma, electropermeabilization


The dependences of the fraction of electroporated mouse hepatoma MH-22A cells on the pulse intensity were obtained for the cells exposed to single square-wave electric pulses with the durations from 95 ns to 20 us. The amplitude of an electric pulse was varied from 0.4 to 12 kV/cm. Increasing the intensity of the electric field pulse increased the fraction of electroporated cells. The electric field which leads to a given percentage of electroporation decreased with increasing the pulse length. The dependence of the amplitude of the electric pulse required to electroporate 50% of mouse hepatoma MH-22A cells on the pulse duration was also determined.
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