Nanotech 2010

CNT and Silver-CNT Nanoparticles provide immune responses against RSV

S. Boyoglu, L. Adiani, C. Boyoglu, M. Mishra, V. Rangari, V. Dennis, S.R. Singh
Alabama State University, US

Keywords: CNT, Ag-CNT, RSV

Abstract:

Nanobiotechnology and its potential to be used in treating and diagnosing general and viral infections have allowed us to step into a new realm, when dealing with Respiratory synctical virus (RSV). RSV has long been considered a major respiratory tract pathogen of children causing lower respiratory tract infections and pneumonia. In the present study, Silver coated MWCNT and MWCNT nanoparticles were evaluated for their cytotoxicity on HEp-2, Vero and J774 macrophage cell lines using the Trypan blue and MTT assays. The cells were incubated with various concentrations of the nanoparticles and assessed for the cell viability at 24, 48, and 72 hours. The results show that at 1.25 µg/ml the cell viability is at 85 % which decreased to about 65 % at 5µg/ml after 48 h post-transfection. Then, HEp-2 and Vero cells were infected with RSV in presence and absence of nanoparticles and inhibition effect of the nanoparticles on RSV was evaluated using immunofluorescence. Additionally, the characteristics of the nanoparticles were evaluated using AFM, TEM. Moreover, the immunological response of the nanoparticles is currently being analyzed using J774 A1 macrophages cell line with ELISPOT assay.
 
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