Nanotech 2010

Analysis of the Relationships Between the Parameters of the Electric Pulse

G. Saulis
Vytautas Magnus University, LT

Keywords: electroporation, nanosecond, erythrocytes, pulse duration

Abstract:

Theoretical relationships between the parameters of the electric pulse which is necessary for the poration of the cell have been obtained. Analysis has been made for the square wave, exponential and sine wave ac electric pulses. At first the relationships between the external electric field strength required to porate cell, Ep, and the length of the square wave and exponential pulses, tau, were studied. It has been obtained that for long pulses the electric field strength required for the poration of cell membranes increases with decreasing pulse length though very slowly but for short pulses the electric field strength required increases rapidly. The relationships between Ep and the frequency of the applied ac field, f, for various ac pulse lengths were calculated taking into account that Vm induced is dependent on the frequency of the applied field. It has been obtained that although the electric field strength for electroporation, Ep, is constant for frequencies tau < 10 kHz, its value depends on the pulse duration: the longer the pulse the lower the value of Ep. At higher frequencies ( tau >10 kHz) Ep is dependent on the frequency of the ac field. Theoretical dependencies are in agreement with experimental ones.
 
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