Nanotech 2010

Intracellular Analysis by SERRS and Nanoparticles

R. Stevenson, L. Senior, J. Alexander, M. Geboers, K. Faulds, D. Graham
University of Strathclyde, UK

Keywords: SERRS, SERS, nanoparticles, intracellular detection

Abstract:

We report the first use of ‘bare’ nanoparticles as both a cellular delivery vehicle and a spectroscopic detection platform to enable surface enhanced resonance Raman scattering (SERRS) detection. We have shown how this approach can be used to observe the conversion of a specific Raman active substrate whose enzyme is naturally occurring within that cell line. The technique has been used to pinpoint unique Raman signals at specific sites within a cell at nm resolution, suggesting a novel approach for enzyme localization and representing a major advancement upon both current SERRS and fluorescence cell based research. Further we show how addition of an enzyme inhibitor can be used to reduce the SERRS output in a dose dependant manner, a significant breakthrough upon current enzyme cellular analysis.
 
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