Nanotech 2010

Spatially nanopatterned ligands alter the response of ErbB receptors (invited presentation)

K. Salaita
Emory University, US

Keywords: dip-pen nanolithography


Cell survival depends on the accurate recognition and response of surface receptors to their immediate chemical environment. The homo- and hetero-association of membrane receptors at multiple length scales is believed to be a key step in the initiation of biochemical signals. For example, the receptor tyrosine kinase ErbB2, which is overexpressed in 20-30% of breast cancers, can be activated by clustering with other ErbB family receptor proteins or through overexpression-driven oligomerization. We investigate the existence and importance of large scale (from 10 nm to 1 micron in scale) ErbB2 associations in living cells using nanopatterned synthetic interfaces. A suite of bottom-up and top-down nanofabrication techniques are used to tailor supported lipid membranes that are decorated with specific ligands. Signaling outputs are measured using a range of bioanalytical tools in order to quantify cellular response to spatially nanopatterned ligands. The results of these investigations will be presented in the context of the ErbB2 signaling pathways in cancer cell lines known to overexpress the receptor.
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