Nanotech 2010

Selection of new breast cancer cell-targeting peptides from landscape phage library

G. Abbineni, S. Modali, B. Safiejko-Mroczka, V.A. Petrenko, C. Mao
The University of Oklahoma, Norman, US

Keywords: nanotechnology, peptides, phage, cancer

Abstract:

We report the identification of SKBR-3 cell-binding peptides by bio-panning with a new type of phage-displayed random peptide library (called landscape phage library) against whole SKBR-3 cells. In the multibillion landscape phage library f8/8, random 8-mer peptides are fused to each of the ~3900 copies of major coat protein (pVIII). The cell-specific binding of the selected peptides to SKBR-3 cancer cells was confirmed by ELISA. The affinity-selected peptides were further analyzed by using REceptor LIgand Contacts (RELIC) program. New SKBR-3 cell-binding peptides are selected using landscape phage libraries. The RELIC/MATCH program was used to align the affinity selected sequences to ErbB family binding proteins. The affinity selected peptides were found to share several common binding motifs with EGF and other erbB family binding proteins. Novel identified cell-targeting peptides can find potential applications in targeted drug- and gene-delivery systems and for imaging, molecular monitoring and profiling of breast cancer.
 
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