Nanotech 2010

Polymer-Grafted-Multi-Walled Carbon Nanotubes (polymer-g-MWNT) for In Vitro Gene Delivery

A. Nunes, K.T. Al-Jamal, N. Amsharov, S. Padmanabhan, M. Burghard, K. Kostarelos
Nanomedicine Lab, UK

Keywords: polymer-grafted CNT, gene delivery, Polyethylenimine, Pollyallylamine, gene expression


the study based on the capability of different cationic polymer-g-MWNTs to efficiently complex and transfect plasmid DNA (pCMV-βGal) without promoting cytotoxicity. Carboxylated MWNT were chemically conjugated to the cationic polymer PEI (polyethylenimine), PAA (polyallylamine) or a mixture of the two polymers. In order to explore the potential of these polymer-g-MWNTs as gene delivery systems, we first studied their capacity to complex pDNA using agarose gel electrophoresis. Gel migration studies confirmed pDNA binding to polymer-g-MWNT at low mass ratios, particularly in the case of PEI-g-MWNT and PEI/PAA-g-CNT constructs. Additionally, PEI-g-MWNT and/or PEI/PAA-g-MWNT revealed an improvement in gene expression when compared to the naked DNA or to the equivalent amounts of PEI polymer alone.
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