Nanotech 2010

Proteoliposome for Tumor-Targeting by Postinsertion of BAM-Homing Molecule Complex

S.Y. Hwang, H.K. Kim, E.K. Lee
Kyungwon University, KR

Keywords: proteoliposome, BAM, targeted delivery, postinsertion


Proteoliposome for Tumor-Targeting by Postinsertion of BAM-Homing Molecule Complex S. Y. Hwang, H. K. Kim, and E. K. Lee Department of Chemical Engineering, Hanyang University, Ansan, Korea and College of Bionanotechnology, Kyungwon University, Seongnam, Korea The objectives were to fabricate the nano-scale, stable liposome, of which the surface was decorated with proteineous and active homing molecules for tumor-specific targeting, and to demonstrate its targetability by imaging technique. Sterically stable, ligand-targeted liposomes were prepared by a postinsertion method using BAM (biological anchor for membrane) as a linker. BAM consists of oleic acid chains at one end that can interact with phospholipids through lipophilic interactions, and NHS ester at the other end to which a protein can be easily conjugated through amine coupling reaction. Both ends are connected by a linear PEG (polyethylene glycol), which provides biocompatibility and stability. We used inert phosphatidylcholine as a liposomal phospholipid, and BAM-homing molecule complex was simply postinserted after a unilamellar liposome (ca. 200 nm in diameter) was made. Epidermal growth factor was used as the homing molecule to target EGF receptor expressed on tumor surface. The selective targeting was confirmed by fluorescence microscopy. To our knowledge, it is the first report of employing the simple postinsertion method to obtain proteoliposome of which the surface was decorated with both PEG moiety and a protein homing molecule. The method is expected to serve as a platform technology for liposome-based, protein-targeting drug delivery system.
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