Nanotech 2010

Cytotoxicity Assessment of Human Lung Alveolar Cell Exposed to Nanoparticles and Polycyclic Aromatic Hydrocarbons Exhausted from Motorcycle

S.M. Chien, Y.J. Huang, C.C.Y. Chuang, I.L. Hsiao, Y.F. Kuo
National Tsing Hua University, TW

Keywords: nanoparticulate, cytotoxicity, particle-PAHs, motorcycle

Abstract:

The potential harmfulness of traffic pollutants, particulate matter (PM) and polycyclic aromatic hydrocarbons (PAHs), were elevated with the increment of motocycles. However, the toxicology of the nanoparticulate and particle-PAHs (p-PAHs) has rarely been evaluated in human lung alveolar cells. In the study, the characteristics of nanoparticulate and PAHs in diluted motorcycle exhaust are investigated at idle, 15 km h-1 and 30 km h-1 speeds, respectively. In addition, the cell cytotoxicity of nanoparticulate and particulate polycyclic aromatic hydrocarbons (p-PAHs) in emission of a four-stroke motorcycle (4 st mc-1) was studied. According to the result of MTT test, we found that the cell viability of A549 exposed to the PM exhausted from idle condition was lower than that from 15 km h-1 and 30 km h-1 speeds. However, the cell survival rate of A549 stimulated by p-PAHs exhausted from the 30 km h-1 test was 15.9%, which was lower than 31.4% (idle) and 20.2% (15 km h-1). It means the p-PAHs might be the main factor to induce the death of cell in the high speed condition.
 
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