Nanotech 2010

Agarose coating improves protein stability during sustained release

E. De Rosa, I. Yadzi, X. Liu, M. Ferrari, E. Tasciotti
UT HSC Houston, US

Keywords: drug delivery, protein stability, microparticle, nanoporous


The advances in drug-delivery systems over recent decades highly improved the effectiviness of drug administration. Biocompatible, biodegradable porous silicon microparticles stable can be modified to provide controlled release and enhanced stability of the loaded/encapsulated bioactive molecule. However, the stability of the loaded/encapsulated bioactive molecules has not been guaranteed so far. In this work we describe an agarose coating that provided long term protection of the bioactive molecule prior to its delivery. Nanoporous silicon microparticles (MPs) designed, fabricated and engineered for drug delivery were loaded with FITC conjugated BSA and coated using several procedures and agarose compositions. Scanning electro microscopy (SEM) and confocal microscopy, FACS and photometric technique were used to characterize and quantify protein release. The optimal agarose concentration and coating procedure to provide protein protection without affecting particle degradation and protein release rate was identified. The protective effect of agarose coating was tested by adding trypsin to the microparticles solution and by studying the stability of the coated BSA through polyacrylamide gel electrophoresis (SDS page) and HPLC analysis. This study demonstrates that agarose coating is a valuable solution against protein instability during long term release.
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