Nanotech 2010

Interactions of Gold Nanoparticle Reference Materials with DNA in Cell-Free and Cell-Containing Systems

B.C. Nelson
National Institute of Standards and Technology, US

Keywords: genotoxicity, gold nanoparticles, reference materials, liquid chromatography/tandem mass spectrometry, DNA damage

Abstract:

The National Institute of Standards and Technology has developed a trio of increasing diameter (10, 30, 60 nm) zero-valency state gold nanoparticle (AuNP) RMs that have been thoroughly characterized in terms of their physical, chemical and electronic properties, but not in terms of their toxicological properties. To support the use of the AuNP RMs as control materials for nanomaterial toxicity studies, it is necessary to evaluate the biological effects of the RMs in relevant experimental systems. The AuNPs were tested for their ability to induce oxidative DNA damage due to direct physical interactions with calf-thymus DNA (ct-DNA) in a cell-free system and for their ability to induce oxidative DNA damage within the cells of a human liver cancer cell system (HepG2). Oxidatively-induced DNA damage was assessed based on the specific detection and sensitive measurement of four modified 2’-deoxynucleosides using isotope-dilution liquid chromatography/tandem mass spectrometry (LC/MS/MS). Based on comparisons with the background lesion levels in matched control samples, significant DNA damage was not observed in either experimental system. These data suggest that the NIST AuNP RMs could potentially serve as appropriate negative-control RMs for in vitro genotoxicity testing platforms involving metal-based NPs.
 
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