Microtech 2011

Microfluidic Neural Cell Chip for Monitoring of PCBs

S. Park, Y-K Kim
Hankyong National University, KR

Keywords: neural cell chip, PC12, dopamine, PCBs, persistent organic pollutant


Rapid and reliable monitoring of persistent organic pollutants (POPs) is very important to prevent their harmful effect on environment. In this study, microfluidic device to detect polychlorinated biphenyls (PCBs), which are well known insecticide, was developed. A neural cell chip was fabricated based on lab-on-a-chip technique. The neural cell chip was composed of two layers, control layer and fluidic layer, which were made by polydimethylsiloxane (PDMS). The control channels in control layer cross the flow channels in fluidic layer. Two layers were placed on a micro electrodes patterned glass wafer. The thin diaphragm between two channels deflects into the flow channel when the control channel is pressurized. This flow control system, called as microvalve system, could control the flows in fluidic layer for the injection of medium, PC12 neural cells, and samples. PC12 cells were incubated in chamber (300 μm × 300 μm × 20 μm) of fluidic layer. The PCBs have harmful affect on the physiological state of PC12 neural cells, and the dopamine production decreased. The dopamine concentration could be measured by cyclic voltammetry (CV). Dopamine concentration was inversely proportional to PCBs concentration, so we could obtain a calibration curve between detection signals and PCBs concentrations. Using this calibration curve, PCBs concentration in sample solutions could be successfully measured by the fabricated microfluidic neural cell chip.

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