Microtech 2011

Fabrication of PEG Hydrogel Microstructures in Microfluidic System for Multicellular Spheroid Formation, Culturing and Analysis

C.-Y. Fu, H.-Y. Chang
National Tsing Hua University, TW

Keywords: microfluidic system, spheroids, PEG hydrogel, bioartificial liver


In this abstract, we describe a novel PEG-based, simple-to-fabricate, U-shaped micro-scale growth chamber, which allows cell trapping and forming into spheroids as well as culturing in the presence of a continuous perfusion flow, a long-term 3D cell culture can be achieved. We believe that it can provide a good platform for many liver spheroid-based studies and applications in the future.

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