Microtech2010 2010

Label-free, Fluorescenece Detection of DNA Nanoparticle Scaffolds

A.J. Kell, S. Tan, I. Charlebois, M. Boissinot, M. LeClerc, B. Simard
National Research Council, CA

Keywords: DNA, label-free detection, nanoparticle, polymeric transduction


We have developed a nanoparticle-based DNA biosensor that can detect unlabelled ssDNA with good sensitivity (picomolar concentrations). The biosensor makes use of the optical changes of a cationic polymer interacting with ssDNA versus that of hybridized DNA to provide a fluorescence signal only when a specific hybridization event occurs (i.e. only when perfectly complementary DNA is present)
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