Microtech2010 2010

Facile Fabrication of an Enzyme Microreactor Using Magnetic Microbeads

X. Liu, R.C. Lo, F.A. Gomez
California State University, Long Beach, US

Keywords: diaphorase, enzyme, magnetic microbeads, microfluidics, microreactor


In this paper, we present a simple method to fabricate an enzyme microreactor using soft lithography and magnetic microbeads. For proof of concept, we performed an enzymatic reaction by flowing non-fluorescent resazurin through diaphorase (DI) (E.C. 1.6.99) covalently attached to magnetic microbeads packed in a PDMS microfluidic chip to convert it to fluorescent resorufin in the presence of nicotinamide adenine dinucleotide phosphate (NADH).
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