Microtech2010 2010

Functionalized Nanopipette: towards single cell immunoassay

P. Actis, O. Jejelowo, N. Pourmand
University of California, Santa Cruz, US

Keywords: nanopipette, immunoassay, label-free, biosensor

Abstract:

Nanopipette technology is capable of detecting and functional analyzing bio-molecules based on difference on their size, shape and electrical charge. This unique label-free biosensor is inexpensive, easy to fabricate and versatile. Nanopipette functionalized with antibodies specific to HT-2 toxin, a food-borne pathogen, showed a limit of detection for HT-2 toxin as low as 100 fg/ml with a dynamic range of 4 logs. We are currently investigating the application of functionalized nanopipette for HPV proteins detection. A highly sensitive nanopipette probe can be precisely positioned at any sub-cellular region, thus paving the way for single cell immunoassay.
 
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