Silica Nanosprings™ as Support Material for Enzyme Based Continuous Flow Reactors

M.G. Norton, D.C. Hyatt, M. Yahvah
Washington State University, US

Keywords: immobilized enzymes, continuous flow chemistry, nanomaterials


We have developed an enzyme-based continuous flow reactor in which enzyme is immobilized on high-surface area silica Nanosprings that have been grown on stainless steel mesh. Stacks of the Nanospring-covered mesh are packed into a flow reactor through which the substrate solution is pumped. Using β-galactosidase from Aspergillus oryzae as a model system we have observed loading densities as high as 0.9g/g Nanosprings. The current reactor configuration is capable of producing > 9 μmol of product per minute at functional flow rates up to 1.0 mL/min (Fig 4). The reactor utilizes ubiquitous lysine residues on the enzyme surface as attachment points, and should thus be generally applicable to other soluble enzymes.