Clean Technology 2010

Influence of High Temperature on a water rich hybrid stationary phases

L. Alkhateeb, R.M. Smith
King Abdual Aziz University, SA

Keywords: hybrid stationary phases, water rich mobile phase, HPLC, Van’t Hoff


The separation of some phenols and steriodes on Hybrid stationary phases and Gemini C18 column by using water rich methanol-water and superheated water as a mobile phase at high temperatures. The separation gave linear and non linear van’t Hoff. Non linear trend was safely assigned as a systematic change in the properties of the Gemini column reflecting the selectivity of the methylene group.
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