Lipid nanoparticles for enhancing immune responses to protein antigens

T. Courant, H.L. Reynaud, A. Hoang, C. Villiers, F. Mittler, I. Texier, P.N. Marche, F.P. Navarro
CEA LETI MINATEC, FR

Keywords: lipid nanoemulsion, nanovaccine, antigen, adjuvant, peptide delivery, protein delivery

Summary:

In the near future, novel vaccines will be needed, particularly nanoparticles-based vaccine formulations for figthing problematic infectious diseases like tuberculosis, AIDS/ HIV or malaria and also cancers. Here, we described the development of versatile chemical method for grafting peptides and proteins onto lipid nanoemulsions. The number of proteins grafted on each nanoparticle can be finely tuned depending on the reaction conditions and the LipidotsĀ® formulation. These particles bearing protein antigens can be further functionalized with targeting moieties directed against immune cells. Preliminary experiments of in vivo immunisation in mice using ovalbumin (OVA) as antigen model seem to indicate an enhancement of immune responses when OVA is associated to lipid nanoparticle formulation compared to complete Freund adjuvant (CFA). Further investigations are ongoing for revealing the full potential of such particles for vaccination